{"id":5830,"date":"2023-03-27T08:05:10","date_gmt":"2023-03-27T06:05:10","guid":{"rendered":"https:\/\/www.jphres.org\/?p=5830"},"modified":"2023-04-02T14:17:27","modified_gmt":"2023-04-02T12:17:27","slug":"possible-correlation-of-electrochemiluminescence-based-numerical-cut-off-index-value-with-concentration-of-anti-sars-cov-2-antibody-is-it-worth-reporting","status":"publish","type":"page","link":"https:\/\/www.jphres.us.com\/index.php\/jphres\/article\/view\/2079\/","title":{"rendered":"Possible correlation of electrochemiluminescence based numerical cut off index value with concentration of anti-SARS-CoV-2 antibody: Is it worth reporting?"},"content":{"rendered":"<div class=\"item doi\"><span class=\"value\"><a href=\"https:\/\/doi.org\/10.4081\/jphr.2021.2079\">https:\/\/doi.org\/10.4081\/jphr.2021.2079<\/a><\/span><\/div>\n<ul class=\"item authors\">\n<li><span class=\"name\"><strong>Shabnum Khawaja<\/strong><br \/>\n<\/span><span class=\"affiliation\">Department of Chemical Pathology, National Institute of Blood disease and Bone Marrow Transplantation (NIBD), Karachi, Pakistan.<br \/>\n<\/span><span class=\"orcid\"><a href=\"https:\/\/orcid.org\/0000-0002-3218-5287\" target=\"_blank\" rel=\"noopener\">https:\/\/orcid.org\/0000-0002-3218-5287<\/a><\/span><\/li>\n<li><span class=\"name\"><strong>Mohammad Asif<\/strong><br \/>\n<\/span><span class=\"affiliation\">Department of Haematology, National Institute of Blood disease and Bone Marrow Transplantation (NIBD), Karachi, Pakistan.<\/span><span class=\"orcid\"><br \/>\n<a href=\"https:\/\/orcid.org\/0000-0003-3497-9651\" target=\"_blank\" rel=\"noopener\">https:\/\/orcid.org\/0000-0003-3497-9651<\/a><\/span><\/li>\n<li><span class=\"name\"><strong>Samina Naz Mukry<\/strong><br \/>\n<\/span><span class=\"affiliation\">Department of Haematology, National Institute of Blood disease and Bone Marrow Transplantation (NIBD), Karachi, Pakistan.<\/span><\/li>\n<li><span class=\"name\"><strong>Tahir Sultan Shaamsi<\/strong><br \/>\n<\/span><span class=\"affiliation\">Department of Haematology, National Institute of Blood disease and Bone Marrow Transplantation (NIBD), Karachi, Pakistan.<\/span><span class=\"orcid\"><br \/>\n<a href=\"https:\/\/orcid.org\/0000-0001-7667-5838\" target=\"_blank\" rel=\"noopener\">https:\/\/orcid.org\/0000-0001-7667-5838<\/a><\/span><\/li>\n<\/ul>\n<div class=\"item abstract\">\n<h3 class=\"label\">ABSTRACT<\/h3>\n<p><em>Background<\/em>: Many laboratories are reporting a numerical cutoff index value (COI) value for most anti-SARS-CoV-2 qualitative tests. These numerical values in patients\u2019 report ultimately created great confusion in the public and physicians, therefore this study was designed to evaluate the correlation of electrochemiluminescence (ECLIA) based numerical COI values with quantitative ELISA of anti-SARS-CoV-2 antibody.<br \/>\n<em>Design and Methods<\/em>: Two hundred and twenty-eight (228) recovered COVID-19 patients were included; their serum samples were analyzed by quantitative ELISA and ECLIA for anti-SARS-COV-2 antibodies.<br \/>\n<em>Results<\/em>: One hundred and seventy-three (75.8%) patients tested positive by ECLIA and ELISA assay and thirty-seven (6.2%) were tested negative by both methods. A weak positive correlation (r=0.37) was found between numerical COI value of ECLIA with ELISA concentration, which was statistically significant with p&lt;0.001. All values were dispersed on scatter plot and there was no significant linear relationship between ECLIA and ELISA assay.<br \/>\n<em>Conclusions<\/em>: As both testing techniques are base upon the same immunological phenomena of detecting antibodies against nucleocapsid protein. We suggest that COI values are not meant to describe the immunity level of the individuals thus the physicians should not consider it as a quantitative value for antibody levels in COVID-19 patients.<\/p>\n<h3 class=\"label\">REFERENCES<\/h3>\n<div class=\"value\">\n<p>WHO. General&#8217;s opening remarks at the media briefing on COVID-19 &#8211; 11 March 2020. World Health Organization, 2020. Accessed: 11 Sep 2020. Available from:\u00a0<a href=\"https:\/\/www.who.int\/dg\/speeches\/detail\/who-director-general-s-opening-remarks-at-the-media-briefing-on-covid-19---11-march-2020\">https:\/\/www.who.int\/dg\/speeches\/detail\/who-director-general-s-opening-remarks-at-the-media-briefing-on-covid-19&#8212;11-march-2020<\/a><\/p>\n<p>Long QX, Tang XJ, Shi QL, et al. Clinical and immunological assessment of asymptomatic SARS-CoV-2 infections. Nat Med 2020;26:1200-4. DOI:\u00a0<a href=\"https:\/\/doi.org\/10.1038\/s41591-020-0965-6\">https:\/\/doi.org\/10.1038\/s41591-020-0965-6<\/a><\/p>\n<p>European Centre for Disease Prevention and Control. Immune responses and immunity to SARS-CoV-2. 2020. Accessed: 28 Sep 2020. Available from:\u00a0<a href=\"https:\/\/www.ecdc.europa.eu\/en\/covid-19\/latest-evidence\/immune-responses\">https:\/\/www.ecdc.europa.eu\/en\/covid-19\/latest-evidence\/immune-responses<\/a><\/p>\n<p>Hick JL, Hanfling D, Wynia MK, et al. Duty to plan: health care, crisis standards of care, and novel coronavirus SARS-CoV-2. National Academy of Sciences, 2020. Available from:\u00a0<a href=\"https:\/\/nam.edu\/duty-to-plan-health-care-crisis-standards-of-care-and-novel-coronavirus-sars-cov-2\/\">https:\/\/nam.edu\/duty-to-plan-health-care-crisis-standards-of-care-and-novel-coronavirus-sars-cov-2\/<\/a>\u00a0DOI:\u00a0<a href=\"https:\/\/doi.org\/10.31478\/202003b\">https:\/\/doi.org\/10.31478\/202003b<\/a><\/p>\n<p>AESKU.Group [Internet]. 6122 SARS-CoV-2 NP IgG. Accessed: 28 Sep 2020. Available from:\u00a0<a href=\"https:\/\/www.aesku.com\/index.php\/diagnostics\/aeskulisa-elisa-test-kits\/infectious-serology-elisa\/item\/398\">https:\/\/www.aesku.com\/index.php\/diagnostics\/aeskulisa-elisa-test-kits\/infectious-serology-elisa\/item\/398<\/a><\/p>\n<p>Silveira MF, Barros AJ, Horta BL, et al. Population-based surveys of antibodies against SARS-CoV-2 in Southern Brazil. Nat Med 2020;26:1196-9. DOI:\u00a0<a href=\"https:\/\/doi.org\/10.1038\/s41591-020-0992-3\">https:\/\/doi.org\/10.1038\/s41591-020-0992-3<\/a><\/p>\n<p>Nisar MI, Ansari N, Amin M, et al. Serial population based serosurvey of antibodies to SARS-CoV-2 in a low and high transmission area of Karachi, Pakistan. medRxiv 2020.<\/p>\n<p>Roche Diagnostics [Internet]. Elecsys\u00aeAnti-SARS-CoV-2. 2020. Accessed: 28 Sep 2020. Available from:\u00a0<a href=\"https:\/\/diagnostics.roche.com\/global\/en\/products\/params\/elecsys-anti-sars-cov-2.html\">https:\/\/diagnostics.roche.com\/global\/en\/products\/params\/elecsys-anti-sars-cov-2.html<\/a><\/p>\n<p>Stites EC, Wilen CB. The interpretation of SARS-CoV-2 diagnostic tests. Med (N Y) 2020;1:78-89.<\/p>\n<p>Hou H, Wang T, Zhang B, et al. Detection of IgM and IgG antibodies in patients with coronavirus disease 2019. Clin Transl Immunology 2020;9:e01136. DOI:\u00a0<a href=\"https:\/\/doi.org\/10.1002\/cti2.1136\">https:\/\/doi.org\/10.1002\/cti2.1136<\/a><\/p>\n<p>Liu X, Wang J, Xu X, et al. Patterns of IgG and IgM antibody response in COVID-19 patients. Emerg Microbes Infect 2020;9:1269-74. DOI:\u00a0<a href=\"https:\/\/doi.org\/10.1080\/22221751.2020.1773324\">https:\/\/doi.org\/10.1080\/22221751.2020.1773324<\/a><\/p>\n<p>Lippi G, Salvagno GL, Pegoraro M, et al. Preliminary evaluation of Roche Cobas Elecsys Anti-SARS-CoV-2 chemiluminescence immunoassay. Clin Chem Lab Med 2020;58:e251-3. DOI:\u00a0<a href=\"https:\/\/doi.org\/10.1515\/cclm-2020-1042\">https:\/\/doi.org\/10.1515\/cclm-2020-1042<\/a><\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>https:\/\/doi.org\/10.4081\/jphr.2021.2079 Shabnum Khawaja Department of Chemical Pathology, National Institute of Blood disease and Bone Marrow Transplantation (NIBD), Karachi, Pakistan. https:\/\/orcid.org\/0000-0002-3218-5287 Mohammad Asif Department of Haematology, National Institute of Blood disease and Bone Marrow Transplantation (NIBD), Karachi, Pakistan. https:\/\/orcid.org\/0000-0003-3497-9651 Samina Naz Mukry Department of Haematology, National Institute of Blood disease and Bone Marrow Transplantation (NIBD), Karachi, &#8230; <a title=\"Possible correlation of electrochemiluminescence based numerical cut off index value with concentration of anti-SARS-CoV-2 antibody: Is it worth reporting?\" class=\"read-more\" href=\"https:\/\/www.jphres.us.com\/index.php\/jphres\/article\/view\/2079\/\" aria-label=\"More on Possible correlation of electrochemiluminescence based numerical cut off index value with concentration of anti-SARS-CoV-2 antibody: Is it worth reporting?\">Read more<\/a><\/p>\n","protected":false},"author":3,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"open","ping_status":"open","template":"","meta":{"footnotes":""},"class_list":["post-5830","page","type-page","status-publish"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.2 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Possible correlation of electrochemiluminescence based numerical cut off index value with concentration of anti-SARS-CoV-2 antibody: Is it worth reporting? - Journal of Public Health Research<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.jphres.us.com\/index.php\/jphres\/article\/view\/2079\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Possible correlation of electrochemiluminescence based numerical cut off index value with concentration of anti-SARS-CoV-2 antibody: Is it worth reporting? - Journal of Public Health Research\" \/>\n<meta property=\"og:description\" content=\"https:\/\/doi.org\/10.4081\/jphr.2021.2079 Shabnum Khawaja Department of Chemical Pathology, National Institute of Blood disease and Bone Marrow Transplantation (NIBD), Karachi, Pakistan. https:\/\/orcid.org\/0000-0002-3218-5287 Mohammad Asif Department of Haematology, National Institute of Blood disease and Bone Marrow Transplantation (NIBD), Karachi, Pakistan. https:\/\/orcid.org\/0000-0003-3497-9651 Samina Naz Mukry Department of Haematology, National Institute of Blood disease and Bone Marrow Transplantation (NIBD), Karachi, ... 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