{"id":4375,"date":"2023-03-29T10:44:39","date_gmt":"2023-03-29T08:44:39","guid":{"rendered":"https:\/\/www.jphres.org\/?p=4375"},"modified":"2023-04-02T13:58:17","modified_gmt":"2023-04-02T11:58:17","slug":"molecular-identification-of-lactic-acid-bacteria-approach-to-sustainable-food-security","status":"publish","type":"page","link":"https:\/\/www.jphres.us.com\/index.php\/jphres\/article\/view\/2508\/","title":{"rendered":"Molecular Identification of Lactic Acid Bacteria Approach to Sustainable food Security"},"content":{"rendered":"
https:\/\/doi.org\/10.4081\/jphr.2021.2508<\/a><\/span><\/div>\n


\nDessy Abdullah<\/strong>
\n<\/span>Research Scholar, Lincoln University College, Wisma Lincoln, No, 12-18, SS 6\/12, Off Jalan Perbandaran,, 47301 Petaling Jaya, Selangor D. E, Malaysia Lecturer, Medical Faculty, Baiturrahmah University Padang, West Sumatera, Indonesia, Indonesia.<\/span><\/p>\n

Sandeep Poddar<\/strong>
\n<\/span>Deputy Vice Chancellor of Research, Lincoln University College, Malaysia.<\/span>
\nhttps:\/\/orcid.org\/0000-0001-9771-877X<\/a><\/span><\/p>\n

Ramesh Prasath Rai<\/strong>
\n<\/span>Lincoln University College, Wisma Lincoln, No, 12-18, SS 6\/12, Off Jalan Perbandaran, 47301 Petaling Jaya, Selangor, Malaysia, Malaysia.<\/span><\/p>\n

Endang Purwati<\/strong>
\n<\/span>Faculty of Animal Science, University of Andalas, Padang, West Sumatera, Indonesia, Indonesia.<\/span><\/p>\n

Nadia Purnama Dewi<\/strong>
\n<\/span>Research Scholar, Lincoln University College, Wisma Lincoln, No, 12-18, SS 6\/12, Off Jalan Perbandaran,, 47301 Petaling Jaya, Selangor, Malaysia Lecturer, Medical Faculty, Baiturrahmah University Padang, West Sumatera, Indonesia, Indonesia.<\/span><\/p>\n

Yudha Endra Pratama<\/strong>
\n<\/span>Doctoral Program, Faculty of Animal Science, University of Andalas, Padang, Indonesia, Indonesia.<\/span><\/p>\n

\n

ABSTRACT<\/h3>\n

Background:<\/em>\u00a0Dadiah is a traditional dish from West Sumatra made from buffalo milk, which is fermented in bamboo tubes and left at room temperature for \u00b12 days. Dadiah is included in the staple food category because it contains Lactic Acid Bacteria (LAB) which has the potential to be a probiotic. This study aims to determine the identification and characterization of LAB from Dadiah from Halaban, Kab. Fifty Cities, West Sumatra.<\/p>\n

Design and Methods:<\/em>\u00a0A survey method was used in this research with a descriptive analysis, Antimicrobial activity testing was done with bacteria\u00a0Escherichia coli<\/em>\u00a0O157,\u00a0Staphylococcus aureus<\/em>,\u00a0Listeria monocytogenes<\/em>, and\u00a0Listeria innocua<\/em>. Molecular identification was done using the 16S rRNA gene.<\/p>\n

Results:<\/em>\u00a0Probiotic candidate test with the best results in testing for resistance to stomach acid at pH3 with the viability of 65.98%, bile salt resistance 0.3%, viability of 54.90% from 2DA isolates. Antimicrobial activity with the best clear zone area results was obtained in 2DA isolates with\u00a0Escherichia coli<\/em>\u00a0O157 test bacteria of 21.16 mm,\u00a0Staphylococcus aureus<\/em>\u00a0with a clear zone area of 23.17 mm,\u00a0Listeria innocua<\/em>\u00a0of 19.24 mm and\u00a0Listeria monocytogenes<\/em>\u00a0with a clear zone area 18.23 mm in 4DA isolate, LAB identification using 16S sRNA gene, results of running PCR base length 1419bp.<\/p>\n

Conclusions<\/em>: Phylogenetic analysis shows that Dadiah of Limapuluh Kota Regency is a kin to\u00a0Lactobacillus plantarum<\/em>. The superiority of identification technology by using 16S rRNA gene only can be conducted if the nucleotide sequence information of the targeted bacteria is known beforehand.<\/p>\n<\/div>\n

\n

 <\/p>\n

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https:\/\/doi.org\/10.4081\/jphr.2021.2508 Dessy Abdullah Research Scholar, Lincoln University College, Wisma Lincoln, No, 12-18, SS 6\/12, Off Jalan Perbandaran,, 47301 Petaling Jaya, Selangor D. E, Malaysia Lecturer, Medical Faculty, Baiturrahmah University Padang, West Sumatera, Indonesia, Indonesia. Sandeep Poddar Deputy Vice Chancellor of Research, Lincoln University College, Malaysia. https:\/\/orcid.org\/0000-0001-9771-877X Ramesh Prasath Rai Lincoln University College, Wisma Lincoln, No, … Read more<\/a><\/p>\n","protected":false},"author":3,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"open","ping_status":"open","template":"","meta":{"footnotes":""},"class_list":["post-4375","page","type-page","status-publish"],"yoast_head":"\nMolecular Identification of Lactic Acid Bacteria Approach to Sustainable food Security - Journal of Public Health Research<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.jphres.us.com\/index.php\/jphres\/article\/view\/2508\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Molecular Identification of Lactic Acid Bacteria Approach to Sustainable food Security - Journal of Public Health Research\" \/>\n<meta property=\"og:description\" content=\"https:\/\/doi.org\/10.4081\/jphr.2021.2508 Dessy Abdullah Research Scholar, Lincoln University College, Wisma Lincoln, No, 12-18, SS 6\/12, Off Jalan Perbandaran,, 47301 Petaling Jaya, Selangor D. E, Malaysia Lecturer, Medical Faculty, Baiturrahmah University Padang, West Sumatera, Indonesia, Indonesia. Sandeep Poddar Deputy Vice Chancellor of Research, Lincoln University College, Malaysia. https:\/\/orcid.org\/0000-0001-9771-877X Ramesh Prasath Rai Lincoln University College, Wisma Lincoln, No, ... 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